Practically anything from GLaDOS. xD
"I've covered wars you know?" - Frank West, Dead Rising.
"Hey, you the one killin' all the crap when people ask? The Catch-a-Ride near Fyrestone is more busted than my momma's girl parts. Really appreciate you takin' a poke at that. The uh, system, not my mom. Hot dog down a Skag den, know what I'm sayin'?" - Scooter, Borderlands.
"War, war never changes." - Fallout (Any) (That quote just makes me shiver is all, I love Fallout <3)
I've been recently playing Ratchet and Clank 3, this quote comes to mind as one that made me giggle:
"Anybody have any idea how to shut down this forcefield?" "Uh, H-26, why don't you try sticking your finger into the main power transformer?" "You sure that's a good idea sir? This thing looks pretty dangerous" "Suck it up 26, you got nothing to worry about! I've got special training in electronic...uh..thingymajiggies." "Okay, here it goes." "H-26? Come back." "Uh, H-26 has been vaporized sir...But the forcefield has been deactivated!" - Rangers, Ratchet and Clank 3
And to end on two that will forever make me squee:
"It's-a me, Mario!" - Mario, Super Mario 64
"It is pitch black. You are likely to be eaten by a grue.
>What is Grue?
The grue is a sinister, lurking presence in the dark places of the earth. Its favorite diet is adventurers, but its insatiable appetite is tempered by its fear of light. No grue has ever been seen by the light of day, and few have survived its fearsome jaws to tell the tale." - Zork I