There are several scenes that will always stick out in my mind, from various movies. Here are a few of my favorites:
Princess Mononoke - One of my all time favorite scenes takes place just after Ashitaka awakens after Shishigami has healed is fatal gunshot wound. At the time he's too weak to chew, and San has to chew his food for him. I'm always moved by the scene, for some reason.
Men in Black - J's try-out for the MIB is one of my favorite comedic sequences ever. The physical comedy present in that film is very understated, but brilliant, and nothing demonstrates it better than J dragging the table over to him to complete the test.
Finally, my favorite film scene of all time:
My Neighbor Totoro - The scene at the bus stop. No scene in any movie has ever given me so much joy. Everything about it: the nerve-racking wait for their father in the rain, the shots of the slow-moving toad. The highlight, for me, is the pure ecstatic expression Totoro adopts when he hears the sound drops of rain from the canopy makes on his umbrella. There are few scenes that will make me smile every time I watch them, this has the distinction of being one of the only scenes where I giggle out loud every time I watch it.