The ones I follow regularly are Questionable Content, Schlock Mercenary, Dominic Deegan, and El Goonish Shive.
I check Order of the Stick, Penny Arcade, XKCD, Awkward Zombie, Exterminatus Now, and Eight-Bit Theater when the mood strikes me.
I used to read a metric asston more (City of Zeroes, 9th Elsewhere, Misfile, Ctrl Alt Delete, Megatokyo, The Call of Whatever, Secret of Mana Theater, Bob and George, Something Positive...). Some I just stopped reading, some I'm waiting for in print form (Megatokyo), and some I'm probably going to pick up again (Misfile).