Kind of... It depends on the game for me.
I always end up playing female characters whenever I have a choice, and it has been virtually impossible for me to play a male in any game where there are significant customization options (aka, RPG's). As for games that force me to play a male... it depends on the game and the character i'd be playing. Basically... the more I am forced into "being a male", and especially if I don't really like the character, the more difficult I find it to play. If a game is constantly reminding me "you are a guy", then I get a bit turned off. On the other hand, if the character is more "neutral" in their behaviors, or that the writing and story are good enough to make me ignore this hang-up of mine, then it works out. If the game is bad or rubs the main character's male-ness in my face, I am probably going to be annoyed. My biggest hang-up of all is being forced to play as a male in an RPG, whereas I can ignore it while playing a shooter or other games where my character is essentially non-existent.
Examples... I have absolutely no problem with being forced to play as a male in Half-Life 2 or pretty much all shooter games where you are a personality-less dude in the military. Deus Ex and Assassin's Creed II made me wary at first, but I turned out to like the main characters enough that being forced to be male ended up not bothering me. On the other hand... Duke Nukem, even if some of the one-liners make me giggle, is a no-go for me. Wrongly perhaps, I have also avoided The Witcher 1/2 because I don't like the idea of playing an RPG as a male.
I'll admit... I probably don't make much sense. It is kind of hard for me to explain. I guess... in summary... the more I can forget about the fact that I am playing a male character, or that I can at least -really- like that character, then the less I am bothered about being forced to play as one.
:3