I read
Gaming:
Penny Arcade: http://www.penny-arcade.com/comic/
Dueling Analogs: http://www.duelinganalogs.com/
VG Cats: http://www.vgcats.com/
NerfNow: http://nerfnow.com/
Hejibits (debatable for videogames, but whatever): http://www.hejibits.com/
Manly Guys doing Manly Things: http://thepunchlineismachismo.com/
Order of the Stick: http://www.giantitp.com/index.html
Awkward Zombie: http://www.awkwardzombie.com/index.php
Stolen Pixels (one on the Escapist, don't think it's updating any more though): http://www.escapistmagazine.com/articles/view/comics/stolen-pixels/8297-Stolen-Pixels-242-Welcome-to-Town
Others:
XKCD: http://xkcd.com/
Hijinks Ensue: http://hijinksensue.com/
Shortpacked: http://www.shortpacked.com/
Cyanide and Happiness: http://www.explosm.net/
Axe Cop: http://axecop.com/index.php/acepisodes
Hipster Hitler: http://hipsterhitler.com/
Dead Philosophers in Heaven: http://www.dead-philosophers.com/
Spud Comics: http://spudcomics.com/
Comic Critics (about comic books): http://comiccritics.com/
Saturday Morning Breakfast Cereal: http://www.smbc-comics.com/
ChainsawSuit: http://chainsawsuit.com/
Not Quite Webcomics:
The Oatmeal: http://theoatmeal.com/
Hyperbole and a Half: http://hyperboleandahalf.blogspot.com/
I also must recommend Geekosystem.com. Fantastic website, not webcomics, but reports on geek news and other interesting things.
Thats the first time I've written them all down, and it's very sad to see.