In the second game, this is the semifinal boss.
She is easily the best boss in the series. The fight is challenging, her weapon is awesome, her theme is awesome, and the effect it has on the Travis, the player character... Two assassins, equal in strengh and mind, dueling under the setting sun for what is probably an empty cause. When she dies, Travis flips out and swears to stop all the pointless killing that the assassins have to go through. It's fucking powerful. Then we get to the final boss, the one that you've been hungering to get vengance on for the whole game, the one that caused all this pointless killing... and it's a little kid in a hovercar.
This guy has 3 forms. The first one, the hovercar, is embarisingly easy save one bit where you have to grapple him to lower his health. The third form is a fucking parade float, and it is just plain lame. But the second form... he turns into some superhero thing, and it's the most annoying fight ever. He can teleport, he can knock you down, he's damn-near unblockable, he has a shit ton of heatlh even on the 3 or four occasions where you can actually hit him, and if you get near a window, EVERY ONE OF HIS ATTACKS can knock you out of the window for an instant kill. I nearly snapped my Wiimote trying to beat this little shit.