For me it just has to be that one from Mafia, where this one guy has evaded so many assassination attempts they pull out all the stops to get him. If this cutscene were in a film it would have stayed with me long after the film had ended- to see something this brilliant in a video game (games usually being pretty crap at storytelling) - it just blew me away. All the little details- especially that tiny eye twitch- brilliant!
http://www.youtube.com/watch?v=lCOpSCQ5i60
Also the Assault in Conker's Bad Fur Day gets a mention because its that rare perfect spoof- take something serious and put a twist on it that nobody really expects (i.e. the massacre of all the cute cartoon animals) to make it downright hilarious- and slightly more disturbing than the source movie itself.
http://www.youtube.com/watch?v=7o6whHaEmZ8
http://www.youtube.com/watch?v=lCOpSCQ5i60
Also the Assault in Conker's Bad Fur Day gets a mention because its that rare perfect spoof- take something serious and put a twist on it that nobody really expects (i.e. the massacre of all the cute cartoon animals) to make it downright hilarious- and slightly more disturbing than the source movie itself.
http://www.youtube.com/watch?v=7o6whHaEmZ8