Space Spoons said:
The only boss I've found disturbing of late is Typon, a boss from Rygar: The Legendary Adventure for the PS2.
It's basically a 5-headed stone dragon with screaming baby heads for faces. It's completely weird, and I sincerely wish I had a picture to show you. My description simply doesn't do it justice.
Yes, I certainly remember that. And I thought I was the only person who remembered that game, You just made my day!
Anyway, I really felt creeped out listening in Bioshock to the Audio Diary labeled, Mind Control Test. Hearing the young boy's cries as he... well not to reveal anything to those who've not played it, a young boy is forced to do something no little boy would want to do. The sound effects don't help either.
I also was actually pretty frightened at the beginning of the last level of the first Condemned. I don't know whether I was just too into it or whether it was the eccellent voice actor for the villan but it just freaked me out a little.
EDIT:
Librarian Mike said:
One that stands out for me is that one from Castlevania: Aria of Sorrow on the GBA. I can't remember its name, but it's a big ball made out of bodies. Gross.
That would be Legion, and I found the moments leading up to the fight (What with the procession of walking corpses, but especially the silence) to be pretty creepy.