Lets see...
The first time Bowser walks onscreen for the first time in Super Mario 64. I was young at the time and I shat a brick.
Watching my starting pokemon evolve for the first time in Blue.
Seeing my main guy in Harvest Moon 64 get married.
When I beat Snowboard Kids 2 for the fist time.
Getting "Jet" rank on every story mission in Jet Grind Radio.(not an easy task)
From StarFox 64,"Don't ever give up, my son." and "You've grown so strong, Fox."
Some moments from Skies of Arcadia, my favorite game:
1. Drachma's story about "Little Jack".
2. Watching Aika throw a pillow so hard at the wall, it hurts you on the other side.
3. Aika kissing Vyse to ease the stress of the upcoming battle in the morning.
4. Seeing Galcian's special attack for the first time. "Kneel...kneel before me..."
5. Watching a previous enemy give her life and ship to destroy a major villain, and seeing their ships collide, hearing his death scream, and then the explosion.
6. The first time visiting the Silver Shrine Palace, and hearing its eerie, music box music.
7. Seeing the last living group of a race sacrifice themselves to atone for previously passing judgment on humanity by destroying the barrier to the final dungeon.
Finding out who both your revered God and your enemy are in Tales of Symphonia.
Fully restoring my first village in Okami.
The first time I heard Saria's song in Ocarina of Time.
Finally getting all the masks in Majora's Mask.
The ending of Link's Awakening, my first Zelda.
There was a basic moment in MGS3 when I was watching my friend play, he tossed an incendiary round, and got caught on fire. He then shouted "I'm on FIIIIRRRRREE!!!" in the most hilarious way possible.
S-ranking every mission on hard mode in Umbrella Chronicles, giving me unlimited ammo for every weapon. I rocket launched everything.
Going to the first galaxy in Super Mario Galaxy.
Completing the Mercenaries minigame in RE4, Wii edition.
Hearing Snake say "Falcon Punch!" in SSBB as well as calling Colonel in the same death cry he does for him.
Hearing government agents in Psychonauts' milkman stage say,"Look at that woman's breasts. They are large."
And the ending for Kingdom Hearts 2. I really liked the final boss, and although the end cutscene made not much sense, I love how they timed the Sanctuary song with Sora returning the charm to Kairi.
Wow. Long post.