On the citidel near the werehouse:
"2 years, 2 YEARS I've been trying to get this refund! I'm not going to stop now!"
-conversation between Slarian and that one human who tried to get a refund from the Turian on the wards in the first game.
Joker - "I fractured my thumb on the mute button but I think I made my point."
Also when you go to recruit Mordin, and he asks for help, I had my Shepard say:
"You know, just once I would like it if someone said 'Yes Shepard, I would love to go with you, you don't need to help with anything!'"
Reminds me from the first one when your on Feros and about to fight the Thorian, and your teamate is saying all you have to do is destroy a plant...
Shepard: "Nothing's ever simple, is it."