Funniest Line In A Game?

[Fallen-Angel Risen-Demon]

Halo 3 grunt on IWHBYD: You killed Flip-yap! Or Yap-flip was he? It was Yap-flip...no, Flip-yap was his brother! Don't tell me I don't know Flip-yap! Flip-yap and I went to nipple academy together and now he's dead!
I morn for Flip-yap...

 

[Miffmoff]

Mass Effect 2 always mnade me chuckle a little

"Sir Isaac Newtons is the baddest son of a ***** in space!"

 

[The_ModeRazor]

"Nothing like a brush with death to... make you not like death much."
Alistair from DAO. He's got a few others, but meh, I can't remember em all.

Also, From Planescape Torment:

Nameless One: I wonder what it was I said that made death reject me.

Still the Nameless One: Well I, for one, plan on discovering the secrets of the multiverse by rubbing cottage cheese on my belly and eating vast quantities of fresh-water fish. Mmm... cheese.

And an exchange between the dual-crossbow wielding Companion Cube and the floating skull:
Nordom: Attention; Morte. I have a question. Do you have a destiny? A purpose?
Morte: Is Annah still wearing clothes?
Nordom: Affirmatory.
Morte: Then the answer is yes.

Another between those two:
Morte: Hey Nordom, calculate the easiest way for me to snuggle with Annah's pillows.
Nordom: Annah, Morte wishes to snuggle with your pillows!

From Morte:
Morte: C'mon, Chief! We're in a building with some of the sexiest chits this side of the multiverse, and you're stopping to talk to *modrons*?

Even a random NPC bystander gets in on the lolz:
Elderly Hive Dweller: [Upon The Nameless One telling her that he is an adventurer] "I'll bet ye've got all *sorts* o' barmy questions!" She mimics your heroic stance: "Greetin's, I have some questions... can ye tell me about this place? Who's the Lady o' Pain? I'm lookin' fer the Magic Girdle o' Swank Iron, have ye seen it? Do ye know where a portal ta the 2,817th Plane o' the Abyss might be? Do ye know where the Holy Flamin' Frost-Brand Gronk-Slayin' Vorpal Hammer o' Woundin' an' Returnin' an' Shootin'-Lightnin'-Out-Yer-Bum is?" She spits. "Dung, all o' it! Only gets ye in the Dead-Book! I ought ta kick ye in the shins fer even pesterin' a poor ol' woman about it all! Now go away an' leaves me in peace!

 

[zane224]

At the End of Dragon Age (well, ONE of the endings) when there is the party at the end Sten is standing in a corner alone. when you talk to him he says "They said there would be cake. There is no cake. The cake is a lie."

 

[DethVanXan]

Behold; The Sea of Black Tears.
Really more of a lake, than a sea.

Brutal Legend, such a funny game. Disagree if you like on that.

 

[zidine100]

forward to sneakification

yaynification later.

or

that's odd, by my calculations, you dont need a bra

or

its so big (this was with a really bad english dub over of persona 2 eternal punishment.)
made me laugh every time.

 

[Joe Matsuda]

I hear it's amazing when the famous purple stuffed worm in flap-jaw space with the tuning fork does a raw blink on Hari-kari rock.

I need scissors!

61!

 

[micky]

When I said "deadly neurotoxin," the "deadly" was in massive sarcasm quotes. I could take a bath in this stuff. Put it on cereal, rub it right into my eyes... honestly, it's not deadly at all... to *me*. You, on the other hand, are going to find its deadliness... a lot less funny. - GlaDOS, i love that game

 

[rowanfield]

Probably something from Psychonauts.
In fact, nearly any line of Psychonauts
Especially the Lungfishopilis scene. Love it.

 

[GrizzlerBorno]

"Why don't you just marry that thing, if you love it so much!
Do you want to marry it?WELL I WON"T LET YOU!... HA, how does that feel?"

 

[robotam]

[Nefarious' brain transmits a soap station]

Lance: Oh Janice, promise me you'll never leave me again for that Englebert.
Janice: Oh Lance!
[kissing]
Janice: you make me feel like a young boy again!
Lance: [pause] Say what?

Ratchet Gladiator. Love that game. Such inappropriate humour.

Anything. Anything from Portal.
And your uncle in Assassins Creed 2. "It's a-me, Mario!"

Another one from Ratchet and Clank, along the same lines as the Assassins Creed one, is when the plumber says

"plumbers don't go diving down strange pipes all willy nilly. That'd be ridiculous!"

 

[Lordmarkus]

Mass effect 2

The newtons second law speech. Anybody who has played the game knows what I mean

Sir Isaac Newton is the deadliest son-of-a-***** in space!

OT: Essentially everything from here:Other golden examples are Mass Effect 2, Fallout series, Grim Fandango, Company of Heroes and Team Fortress 2.

 

[MasTerHacK]

Dammit Fisher, this isn't a video-game!

 

[YoBadMama]

"You were almost a Jill sandwich!"

 

[Diligent]

Giants: Citizen Kabuto had some good lines, but it's been so long I can't remember much.
Also Portal.
But I enjoy:

"Hold it Snake, time to change the disk. I know, I know it's a pain. But you need to swap disk 1 for disk 2. You see the disk labeled 2?"
"Uuh, no."
"Huh? Oh, wait! We're on Playstation 3. It's a Blu-Ray disk - dual layered too. No need to swap."
"Damn it Otacon get a grip!"

 

[robotam]

The commentary from the PS2 era Pro Evolution Soccer games.
Oh, it was so bad.

It was terrible, but the commentary in some of the old Smackdown games were worse.
"And the fans are singing a chat directed at the competitors in the ring"
"If one of these superstars hits their finisher, there is a chance the match will be over".

 

[Nopraptor]

For me it's when CJ says Coolio in San Andreas it's really funny when you try to say it like a gangster

 

[Jester00]

"Why don't you just marry that thing, if you love it so much!
Do you want to marry it?WELL I WON"T LET YOU!... HA, how does that feel?"

epic

 

[snappydog]

There was a bit in Mass Effect 2 where Shepard could do the Renegade action of pushing somebody out of a window... I'm sure it would have made sense in context, but what actually happened was the guy said something like 'Just let me -' at which point I killed him, and Shepard went 'How 'bout goodbye.' Which I found funny. I liked the idea of him spouting nonsensical sayings after killing people - just stab somebody in the face and go 'When in Rome' and walk off or something.

 

[Phoenix09215]

No warcraft 3 quotes yet? Very well...

-"My blood cries out for the vengeance of my people's blood, which
can only be repaid with twice as much blood! Or maybe three times
as much blood! Like, if you went to hell and it was full of blood,
and that blood was on fire, and it was raining blood, then maybe
THAT would be enough blood. But, uh... probably not." (Blood Mage)

-"This... is... my... BOOMSTICK!" (Rifleman)

-"Crouch tiger! I sense a hidden dragon." (Preistess of the Moon)

Theres plenty more though... http://www.gamefaqs.com/mac/918256-warcraft-iii-the-frozen-throne/faqs/18136 theres the link if you would like to see the rest!