There's the classic "Jill sandwich" quote from Resident Evil.
But I like "I should have been the one that brought your dark soul back into the light!" because the actor's voice cracks on the final word--which then echoes around the empty chamber in which the dramatic scene is taking place. (Devil May Cry, I think).
Plus there's "I am the master! I am the fucking universe!" from one of the Alone in the Dark games.
But who can beat President Bush?
"Childrens do learn when standards are high"
"Rarely is the question said, are--is our children learning?"